![All lanes : Anti-Cannabinoid Receptor I (phospho S316) antibody [EPR2223(N)] (ab186428) at 1/1000 dilutionLane 1 : Human fetal brain lysateLane 2 : Human fetal brain lysate with Lambda phosphataseLysates/proteins at 1/10 dilution per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/20224_ab186428-219963-ab186428.jpg)
All lanes : Anti-Cannabinoid Receptor I (phospho S316) antibody [EPR2223(N)] (ab186428) at 1/1000 dilutionLane 1 : Human fetal brain lysateLane 2 : Human fetal brain lysate with Lambda phosphataseLysates/proteins at 1/10 dilution per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow cytometry analysis of Cannabinoid Receptor I (phospho S316) expression in 2% paraformaldehyde-fixed SH-SY5Y cells using ab186428 at 1/200 dilution (red) or a rabbit IgG isotype control (green), SH-SY5Y cells preincubated with Cannabinoid Receptor I (phospho S316) peptide (blue) or non-phosopho Cannabinoid Receptor I (phospho S316) peptide (orange). FITC-conjugated goat anti-rabbit IgG (1/150 dilution) was used as the secondary antibody.