Anti-Cannabinoid Receptor I antibody

• Supplier: Abcam
• Catalog: ab23703
• Prices: $400.00
• Sizes: 150 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IHC-P IHC-F ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Dog, Human, Bovine, Chimpanzee, Monkey
• Antigen: Synthetic peptide: MSVSTDTSAE AL , corresponding to C terminal amino acids 461-472 of Human Cannabinoid Receptor I(Peptide available as ab50542
• Blocking Peptide: Cannabinoid Receptor I peptide
• Description: Rabbit Polyclonal
• Gene: CNR1
• Conjugate: Unconjugated

Product images

Anti-Cannabinoid Receptor I antibody (ab23703) + Human cerebellum at 30 µg

Immunoperoxidase staining of CA region of rat hippocampus with the Cannabinoid Receptor I antibody (ab23703, 4ug/ml). the majority of the protein is localized in the pre-synaptic axons, shown as brown particulaes. A few neurons also show cytoplasm staining.

ab23703 staining Cannabinoid Receptor I in rat brain tissue by Immunohistochemistry (Frozen sections). After fixation in 4% PFA in PBS (pH 7.4), brains were cryoprotected in 30% sucrose in PBS at +4°C, frozen, and thereafter kept at -80°C until used. For the immunostaining, brains were cryosectioned in 30 µm slices, collected in PBS (pH 7.4), and processed in a free-floating system. Slices were first incubated in the blocking solution (BS) containing 2% bovine serum albumin, 2% goat serum, and 0.1% Triton X-100 in PBS (pH 7.4) for 1 hour at room temperature, and thereafter with the primary antibody for 24–48 hours at +4°C in BS at a 1/1000 dilution. After washing in PBS, slices were incubated with the biotin-conjugated secondary antibody (1/4000) in BS, rinsed with PBS, and incubated with the avidin-peroxidase conjugate in BS for 1 hour at room temperature. The staining was detected using 3,3-diaminobenzidinetetrahydrochloride as a chromogen.

Immunofluorescence analysis of mouse cultured hippocampal neurons, staining Cannabinoid Receptor I with ab23703. Cells were fixed with paraformaldehyde, permeabilized with 0.05% Saponin and blocked with 10% fetal calf serum for 2 hous at 4°C. Samples were incubated with primary antibody (1/200 in 0.05% Saponin + fetal calf serum) for 12 hours at 23°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody. See Abreview

Product References

Potential upstream regulators of cannabinoid receptor 1 signaling in prostate - Potential upstream regulators of cannabinoid receptor 1 signaling in prostate

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Cannabinoid receptor type 1 and 2 expression in the skin of healthy dogs and dogs - Cannabinoid receptor type 1 and 2 expression in the skin of healthy dogs and dogs

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A high cannabinoid CB(1) receptor immunoreactivity is associated with disease - A high cannabinoid CB(1) receptor immunoreactivity is associated with disease

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