ICC/IF image of ab96354 stained PANC-1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96354 at 5 ug/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-CAP1 antibody (ab96354) at 1/1000 dilutionLane 1 : Hep G2 whole cell lysateLane 2 : Raji whole cell lysateLysates/proteins at 30 µg per lane.
ab96354 at 1/500 dilution staining paraffin-embedded DLD1 xenograft