All lanes : Anti-Carbonic Anhydrase IX antibody (ab184006) at 1/1000 dilutionLane 1 : HepG2 whole cell lysate, untreatedLane 2 : HepG2 whole cell lysate, treated with 200 µM CoCl2 for 24 hrLysates/proteins at 30 µg per lane.
Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling Carbonic Anhydrase IX with ab184006 at 1/500 dilution.
Immunofluorescent analysis of A431 cells labeling Carbonic Anhydrase IX with ab184006 at 1/500 dilution. A431 cells were fixed in ice-cold MeOH for 5 min. Cells were treated with 200 μM CoCl2 (right) or untreated (left) Green: Carbonic Anhydrase IX protein showing membrane staining.Blue: Hoechst 33342.