All lanes : Anti-Carboxypeptidase B antibody - N-terminal (ab170724) at 1/100 dilutionLane 1 : K562 cell lysateLane 2 : Hela cell lysateLysates/proteins at 35 µg per lane.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling Carboxypeptidase B with ab170724 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of HeLa cells labeling Carboxypeptidase B with ab170724 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of HeLa cells labeling Carboxypeptidase B with ab170724 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.