![Peptide Competition and Phosphatase TreatmentLysates prepared from HeLa cells were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda (λ) phosphatase (5), blocked with a 3% Milk-TBST buffer for one hour at room temperature, and incubated with CK2β [pS209] (ab12861) antibody for two hours at room temperature in a 3% Milk- TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphoserine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method. The data show that only the peptide corresponding to CK2β [pS209] blocks the signal, verifying the specificity of the antibody.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/20982_ab12861v2.jpg)
Peptide Competition and Phosphatase TreatmentLysates prepared from HeLa cells were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda (λ) phosphatase (5), blocked with a 3% Milk-TBST buffer for one hour at room temperature, and incubated with CK2β [pS209] (ab12861) antibody for two hours at room temperature in a 3% Milk- TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphoserine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method. The data show that only the peptide corresponding to CK2β [pS209] blocks the signal, verifying the specificity of the antibody.