Anti-Caspase-14 antibody
| Name | Anti-Caspase-14 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab45415 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to Human Caspase-14 (C terminal) |
| Blocking Peptide | Caspase-14 peptide |
| Description | Rabbit Polyclonal |
| Gene | CASP14 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab45415 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab45415 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5min) HeLa cells at 5ug/ml.
All lanes : Anti-Caspase-14 antibody (ab45415) at 1 µg/mlLane 1 : Jurkat cell lysate Lane 2 : Jurkat cell lysate + blocking peptideSecondaryanti-rabbit IgG