Anti-Caspase-14 antibody
| Name | Anti-Caspase-14 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab139741 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB |
| Species Reactivities | Human, Mouse, Rat, Chimpanzee, Monkey, Ape |
| Antigen | Synthetic peptide corresponding to Human Caspase-14 aa 200 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Rabbit Polyclonal |
| Gene | CASP14 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of Caspase-14 staining in Human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab139741, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-Caspase-14 antibody (ab139741) at 1 µg/ml + Skin (Human) Tissue Lysate - adult normal tissue (ab30166) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.