Anti-Caspase-2L antibody
| Name | Anti-Caspase-2L antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2251 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-F ICC/IF ICC/IF IHC-P IP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to Human Caspase-2 aa 300-400 conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Blocking Peptide | Human Caspase-2L peptide |
| Description | Rabbit Polyclonal |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of Caspase-2 staining in human hippocampus FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2251, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ICC/IF image of ab2251 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2251, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, anti-rabbit DyLight® 488 used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-Caspase-2L antibody (ab2251) at 1 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 2 : HEK293 Human embryonic kidney cell line Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![Caspase 2 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Caspase 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab2251.Secondary: Mouse monoclonal [SB62a] Secondary Antibody anti-Rabbit IgG light chain HRP (ab99697).Band: 35kDa: Caspase 2 .](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/21171_active-Caspase-2-Primary-antibodies-ab2251-8.jpg)
Caspase 2 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Caspase 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab2251.Secondary: Mouse monoclonal [SB62a] Secondary Antibody anti-Rabbit IgG light chain HRP (ab99697).Band: 35kDa: Caspase 2 .
Lane 1 : MarkerLanes 2 - 7 : Anti-Caspase-2L antibody (ab2251) at 1 µg/mlLane 1 : As aboveLane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab27251) at 20 µgLane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µgLane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909) at 20 µgLane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899) at 20 µgLane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902) at 20 µgLane 7 : Recombinant Caspase 2 antibody at 10 µgSecondaryLanes 2 - 7 : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilutionPerformed under reducing conditions.
Product References
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