Anti-Caspase-8 antibody

• Supplier: Abcam
• Catalog: ab25901
• Prices: $390.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-P ICC/IF WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to Human Caspase-8 (C terminal)
• Blocking Peptide: Human Caspase-8 peptide
• Description: Rabbit Polyclonal
• Gene: CASP8
• Conjugate: Unconjugated

Product images

Lane 1 : Anti-Caspase-8 antibody (ab25901) at 0.5 µg/mlLane 2 : Anti-Caspase-8 antibody (ab25901) at 1 µg/mlLane 3 : Anti-Caspase-8 antibody (ab25901) at 2 µg/mlLane 1 : Jurkat cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : Jurkat cell lysate.

Anti-Caspase-8 antibody (ab25901) at 1 µg/ml + Active human Caspase-8 full length protein (ab54117) at 1/0.01 dilutionSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ab25901 at 2ug/ml staining Caspase-8 in Jurkat cells by ICC/IF

IHC image of Caspase-8 staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25901, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

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