Anti-Caspr antibody [S65-35]
| Name | Anti-Caspr antibody [S65-35] |
|---|---|
| Supplier | Abcam |
| Catalog | ab105571 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | S65-35 |
| Applications | FC WB IHC-P IHC-F ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Fusion protein: QNHRYKGSYH TNEPKATHDS HPGGKAPLPP SGPAQAPAPT PAPTQVPTPA PAPASGPGPR DQNLPQILEE SRSE, corresponding to C terminal (cytoplasmic domain) amino acids 1308-1381 of Rat Caspr (P97846) |
| Description | Mouse Monoclonal |
| Gene | CNTNAP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab105571 staining Caspr in Mouse brain tissue sections by Immunohistochemistry.
ab105571 staining Caspr in Mouse brain tissue sections by Immunohistochemistry.
![Anti-Caspr antibody [S65-35] (ab105571) at 1/1000 dilution + Rat brain membrane tissue lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/21428_ab105571-199558-ab105571wb.jpg)
Anti-Caspr antibody [S65-35] (ab105571) at 1/1000 dilution + Rat brain membrane tissue lysate
![Overlay histogram showing SH-SY5Y cells stained with ab105571 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab105571, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/21429_ab105571-1-ab105571FC.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab105571 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab105571, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Product References
Early proliferation does not prevent the loss of oligodendrocyte progenitor cells - Early proliferation does not prevent the loss of oligodendrocyte progenitor cells
Payne SC, Bartlett CA, Savigni DL, Harvey AR, Dunlop SA, Fitzgerald M. PLoS One. 2013 Jun 11;8(6):e65710.
Central nervous system remyelination in culture--a tool for multiple sclerosis - Central nervous system remyelination in culture--a tool for multiple sclerosis
Zhang H, Jarjour AA, Boyd A, Williams A. Exp Neurol. 2011 Jul;230(1):138-48.