Anti-CAT1 antibody
| Name | Anti-CAT1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab37588 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F WB |
| Species Reactivities | Mouse, Human, Orangutan |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human CAT1 (gene: SLC7A1) |
| Description | Rabbit Polyclonal |
| Gene | SLC7A1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-CAT1 antibody (ab37588) at 1 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ICC/IF image of ab37588 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab37588, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
IHC-FoFr image of CAT1 staining on Mouse Spinal Cord sections using ab37588 (1:1000). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the spinal cord were cryoprotected in sucrose 30% overnight. Spinal cords were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.See Abreview
Product References
Functional role of arginine during the peri-implantation period of pregnancy. II. - Functional role of arginine during the peri-implantation period of pregnancy. II.
Wang X, Frank JW, Xu J, Dunlap KA, Satterfield MC, Burghardt RC, Romero JJ, Hansen TR, Wu G, Bazer FW. Biol Reprod. 2014 Sep;91(3):59.
Nutritional stress and arginine auxotrophy confer high sensitivity to chloroquine - Nutritional stress and arginine auxotrophy confer high sensitivity to chloroquine
Battisti S, Valente D, Albonici L, Bei R, Modesti A, Palumbo C. Am J Respir Cell Mol Biol. 2012 Apr;46(4):498-506.
Arginine transport in human monocytic leukemia THP-1 cells during macrophage - Arginine transport in human monocytic leukemia THP-1 cells during macrophage
Barilli A, Rotoli BM, Visigalli R, Bussolati O, Gazzola GC, Dall'Asta V. J Leukoc Biol. 2011 Aug;90(2):293-303.