Anti-Catalase antibody [EP1929Y] - Peroxisome Marker

• Supplier: Abcam
• Catalog: ab76024
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1929Y
• Applications: WB ICC/IF FC IHC-P
• Species Reactivities: Mouse, Human
• Antigen: A synthetic peptide corresponding to residues near the C terminus of human Catalase
• Description: Rabbit Monoclonal
• Gene: CAT
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab76024 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76024, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024) at 1/20000 dilution + HeLa cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical staining of Catalase in paraffin embedded human normal brain tissue using ab76024 at a 1/100 dilution.

Product References

Hydrogen peroxide stimulates the epithelial sodium channel through a - Hydrogen peroxide stimulates the epithelial sodium channel through a

Ma HP. J Biol Chem. 2011 Sep 16;286(37):32444-53.

Mesenchymal stem cells restore frataxin expression and increase hydrogen peroxide - Mesenchymal stem cells restore frataxin expression and increase hydrogen peroxide

Kemp K, Mallam E, Hares K, Witherick J, Scolding N, Wilkins A. PLoS One. 2011;6(10):e26098.