Anti-Cathepsin D antibody [EPR3057Y]
| Name | Anti-Cathepsin D antibody [EPR3057Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab75852 |
| Prices | $397.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR3057Y |
| Applications | WB IP IHC-P ICC/IF FC |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Cathepsin D aa 350 to the C-terminus (C terminal) |
| Description | Rabbit Monoclonal |
| Gene | CTSD |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-Cathepsin D antibody [EPR3057Y] (ab75852) at 1/10000 dilutionLane 1 : MCF-7 cell lysateLane 2 : A431 cell lysateLane 3 : SKBR3 cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP-labeled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/21583_ab75852wb.jpg)
All lanes : Anti-Cathepsin D antibody [EPR3057Y] (ab75852) at 1/10000 dilutionLane 1 : MCF-7 cell lysateLane 2 : A431 cell lysateLane 3 : SKBR3 cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP-labeled goat anti-rabbit at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab75852 at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded human liver tissue using ab75852 at 1/500 dilution.
Overlay histogram showing MCF-7 cells stained with ab75852 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75852, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave no signal in MCF-7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
Persistence versus escape: Aspergillus terreus and Aspergillus fumigatus employ - Persistence versus escape: Aspergillus terreus and Aspergillus fumigatus employ
Slesiona S, Gressler M, Mihlan M, Zaehle C, Schaller M, Barz D, Hube B, Jacobsen ID, Brock M. PLoS One. 2012;7(2):e31223.
Novel proteomic strategy reveal combined alpha1 antitrypsin and cathepsin D as - Novel proteomic strategy reveal combined alpha1 antitrypsin and cathepsin D as
Xie LQ, Zhao C, Cai SJ, Xu Y, Huang LY, Bian JS, Shen CP, Lu HJ, Yang PY. J Proteome Res. 2010 Sep 3;9(9):4701-9.