Anti-Caveolin-3 antibody - Caveolae Marker
| Name | Anti-Caveolin-3 antibody - Caveolae Marker |
|---|---|
| Supplier | Abcam |
| Catalog | ab30750 |
| Prices | $394.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF IP IHC-P WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to Human Caveolin-3 aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Rabbit Polyclonal |
| Gene | CAV3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Caveolin-3 antibody - Caveolae Marker (ab30750) at 1 µg/mlLane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 2 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)Lane 4 : Skeletal Muscle (Rat) Tissue Lysate - normal tissue (ab29376)Lysates/proteins at 20 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab30750 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30750, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Caveolin 3 - Caveolae Marker was immunoprecipitated using 0.5mg Mouse skeletal muscle whole tissue extract, 5µg of Rabbit polyclonal to Caveolin 3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse skeletal muscle whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab30750.Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.Band: 17kDa: Caveolin 3 - Caveolae Marker.
Product References
Confinement of beta(1)- and beta(2)-adrenergic receptors in the plasma membrane - Confinement of beta(1)- and beta(2)-adrenergic receptors in the plasma membrane
Valentine CD, Haggie PM. Mol Biol Cell. 2011 Aug 15;22(16):2970-82.
Inhibition of mammalian target of rapamycin (mTOR) signalling in C2C12 myoblasts - Inhibition of mammalian target of rapamycin (mTOR) signalling in C2C12 myoblasts
Willett M, Cowan JL, Vlasak M, Coldwell MJ, Morley SJ. Cell Signal. 2009 Oct;21(10):1504-12.