Flow cytometric analysis of ab76002 at 1/50 dilution staining permeabilized Jurkat cells, untreated (green) or pervanadate-treated (red) and pervanadate-treated Jurkat cells preincubated with immunising phospho-peptide (blue) or non-phospho peptide (orange).
All lanes : Anti-CBL (phospho Y700) antibody [EP2225Y] (ab76002) at 1/10000 dilutionLane 1 : Jurkat cell lysate, untreatedLane 2 : Jurkat cell lysate, treated with pervanadateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution