Anti-cbx7 antibody - ChIP Grade
| Name | Anti-cbx7 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab21873 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF ChIP WB IHC-P |
| Species Reactivities | Mouse, Human, Rat, Bovine, Chimpanzee |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human cbx7 |
| Blocking Peptide | Human cbx7 peptide |
| Description | Rabbit Polyclonal |
| Gene | CBX7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-cbx7 antibody - ChIP Grade (ab21873) at 1 µgLane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate (ab14660)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab21873 (1/1000) staining cbx7 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.See Abreview
IHC image of cbx7 staining in Human normal prostate formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21873, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-cbx7 antibody - ChIP Grade (ab21873) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate (ab14660)Lysates/proteins at 10 µg per lane.SecondaryGoat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
Product References
CBX7 modulates the expression of genes critical for cancer progression. - CBX7 modulates the expression of genes critical for cancer progression.
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DNA methylation associated with polycomb repression in retinoic acid receptor - DNA methylation associated with polycomb repression in retinoic acid receptor
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Alu elements in ANRIL non-coding RNA at chromosome 9p21 modulate atherogenic cell - Alu elements in ANRIL non-coding RNA at chromosome 9p21 modulate atherogenic cell
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Altered expression of polycomb group genes in glioblastoma multiforme. - Altered expression of polycomb group genes in glioblastoma multiforme.
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Nonoverlapping functions of the Polycomb group Cbx family of proteins in - Nonoverlapping functions of the Polycomb group Cbx family of proteins in
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Expression of microRNA and their gene targets are dysregulated in preinvasive - Expression of microRNA and their gene targets are dysregulated in preinvasive
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Increased expression of miR-421 in human gastric carcinoma and its clinical - Increased expression of miR-421 in human gastric carcinoma and its clinical
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Role for the MOV10 RNA helicase in polycomb-mediated repression of the INK4a - Role for the MOV10 RNA helicase in polycomb-mediated repression of the INK4a
El Messaoudi-Aubert S, Nicholls J, Maertens GN, Brookes S, Bernstein E, Peters G. Nat Struct Mol Biol. 2010 Jul;17(7):862-8.
Polycomb CBX7 directly controls trimethylation of histone H3 at lysine 9 at the - Polycomb CBX7 directly controls trimethylation of histone H3 at lysine 9 at the
Li Q, Wang X, Lu Z, Zhang B, Guan Z, Liu Z, Zhong Q, Gu L, Zhou J, Zhu B, Ji J, Deng D. PLoS One. 2010 Oct 29;5(10):e13732.