Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121282 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemical analysis of formalin fixed, paraffin embedded Human esophagus tissue labelled with ab121282 at 1/40 dilution.
All lanes : Anti-CCDC23 antibody (ab121282) at 1/250 dilutionLane 1 : MarkerLane 2 : Vector transfected HEK293T cell lysateLane 3 : HEK293T cell lysate transfected with CCDC23 and C-terminal myc-DDK tag