Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, cytoplasm and centrosome. Recommended concentration of ab122527 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-CCDC60 antibody (ab122527) at 1/250 dilutionLane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2 : RT-4 Lane 3 : U-251 MG Lane 4 : Human plasma Lane 5 : liverLane 6 : tonsil
Immunohistochemical staining of CCDC60 in paraffin embedded Human bronchus tissue by Immunohistochemistry, using ab122527 at a 1/300 dilution. The image shows strong cytoplasmic positivity in goblet cells of respiratory epithelial cells.