Immunofluorescent staining of Human cell line U-251 MG shows no positivity. Recommended concentration of ab122550 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122550, at 1:200, staining CCDC89 in Human pancreas tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].
All lanes : Anti-CCDC89 antibody (ab122550) at 1/250 dilutionLane 1 : Negative control (vector only transfected HEK293T lysate) Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal proprietary tag (~3.1 kDa) in mammalian HEK293T cells