Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab130453 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
ab130453, at a dilution of 1/500, staining CCDC97 in paraffin-embedded Human testis tissue by Immunohistochemistry.