Anti-CCL27 antibody [EPR8582(2)]

• Supplier: Abcam
• Catalog: ab186421
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR8582(2)
• Applications: WB IHC-P ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CCL27 aa 50 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: CCL27
• Conjugate: Unconjugated

Product images

Anti-CCL27 antibody [EPR8582(2)] (ab186421) at 1/5000 dilution + Human CCL27 recombinant protein at 0.01 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling CCL27 with ab186421 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Immunohistochemical analysis of paraffin-embedded Mouse skin tissue labeling CCL27 with ab186421 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling CCL27 with ab186421 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi.

Flow cytometric analysis of 2% paraformaldehyde-fixed HACAT cells labeling CCL27 with ab186421 at 1/320 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.