Anti-CCN1 antibody
| Name | Anti-CCN1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab24448 |
| Prices | $398.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-F IHC-P IHC-F WB |
| Species Reactivities | Mouse, Rat, Bovine, Human |
| Antigen | Synthetic peptide corresponding to Mouse CCN1 |
| Blocking Peptide | CCN1 peptide |
| Description | Rabbit Polyclonal |
| Gene | CYR61 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-CCN1 antibody (ab24448) at 1 µg/mlLane 1 : Jurkat cell lysateLane 2 : Mouse small intestine tissue lysateLysates/proteins at 30 µg per lane.
ab24448 staining Human myometrium. Staining can be observed in the cytoplasm and nuclear compartments.Left panel: with primary antibody at 2ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ICC/IF image of ab24448 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24448, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Glomerular cell cross-talk influences composition and assembly of extracellular - Glomerular cell cross-talk influences composition and assembly of extracellular
Byron A, Randles MJ, Humphries JD, Mironov A, Hamidi H, Harris S, Mathieson PW, Saleem MA, Satchell SC, Zent R, Humphries MJ, Lennon R. J Am Soc Nephrol. 2014 May;25(5):953-66.
Role of beta-catenin-regulated CCN matricellular proteins in epithelial repair - Role of beta-catenin-regulated CCN matricellular proteins in epithelial repair
Zemans RL, McClendon J, Aschner Y, Briones N, Young SK, Lau LF, Kahn M, Downey GP. Am J Physiol Lung Cell Mol Physiol. 2013 Mar 15;304(6):L415-27. doi:
Temozolomide modifies caveolin-1 expression in experimental malignant gliomas in - Temozolomide modifies caveolin-1 expression in experimental malignant gliomas in
Bruyere C, Abeloos L, Lamoral-Theys D, Senetta R, Mathieu V, Le Mercier M, Kast RE, Cassoni P, Vandenbussche G, Kiss R, Lefranc F. Transl Oncol. 2011 Apr 1;4(2):92-100.
A role for cysteine-rich 61 in the angiogenic switch during the estrous cycle in - A role for cysteine-rich 61 in the angiogenic switch during the estrous cycle in
Zhang B, Tsang PC, Pate JL, Moses MA. Biol Reprod. 2011 Aug;85(2):261-8.
.
Transcriptome profiling and network pathway analysis of genes associated with - Transcriptome profiling and network pathway analysis of genes associated with
Kang CJ, Chen YJ, Liao CT, Wang HM, Chang JT, Lin CY, Lee LY, Wang TH, Yen TC, Shen CR, Chen IH, Chiu CC, Cheng AJ. Cancer Lett. 2009 Nov 1;284(2):131-40.
A combinatorial role for NFAT5 in both myoblast migration and differentiation - A combinatorial role for NFAT5 in both myoblast migration and differentiation
O'Connor RS, Mills ST, Jones KA, Ho SN, Pavlath GK. J Cell Sci. 2007 Jan 1;120(Pt 1):149-59. Epub 2006 Dec 12.
Effect of VEGF-A on expression of profibrotic growth factor and extracellular - Effect of VEGF-A on expression of profibrotic growth factor and extracellular
Kuiper EJ, Hughes JM, Van Geest RJ, Vogels IM, Goldschmeding R, Van Noorden CJ, Schlingemann RO, Klaassen I. Invest Ophthalmol Vis Sci. 2007 Sep;48(9):4267-76.