Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma (left) breast carcinoma (center) and ovarian carcinoma (right) tissues labelling CCNK with ab85854 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-CCNK antibody (ab85854) at 0.1 µg/mlLane 1 : HeLa whole cell lysateLane 2 : 293T whole cell lysateLane 3 : Jurkat whole cell lysateLysates/proteins at 50 µg per lane.developed using the ECL technique
All lanes : Anti-CCNK antibody (ab85854) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgdeveloped using the ECL technique
IHC image of CCNK staining in Human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85854, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.