ab11466 staining CCR5 in Cynomolgus monkey peripheral blood mononuclear cells by Flow Cytometry. The sample was incubated with the primary antibody (1/100 in PBS + 0.5% BSA) for 1 hour at 4°C.Gating Strategy: Live lymphocytes.See Abreview
Naive T cells from healthy donor were Th1 derived. The gating strategy used was for alive cells. ab11466 was used at a 1/100 dilution.See Abreview
Flow Cytometry analysis of murine splenocytes using ab11466 at a 1/100 dilution.See Abreview
ab11466 staining CCR5 in murine spleen tissue by Immunohistochemistry (Frozen sections).Tissue was fixed with acetone, blocked using 5% goat serum for 1 hour at 25°C and then incubated with ab11466 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rat polyclonal used at a 1/400 dilution.See Abreview