All lanes : Anti-CCR5 antibody [mAbcam 64620] (ab64620) at 10 µg/mlLane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate Lane 4 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.