![Overlay histogram showing Jurkat cells stained with ab68131 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68131, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed Jurkat cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/22846_CCR6-Primary-antibodies-ab68131-1.jpg)
Overlay histogram showing Jurkat cells stained with ab68131 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68131, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed Jurkat cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
![Anti-CCR6 antibody [4C6] (ab68131) at 1/500 dilution + cell lysates of Raji at 30 µgSecondaryGoat anti-mouse secondary antibody conjugated to HRPdeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/22845_ab68131wb.jpg)
Anti-CCR6 antibody [4C6] (ab68131) at 1/500 dilution + cell lysates of Raji at 30 µgSecondaryGoat anti-mouse secondary antibody conjugated to HRPdeveloped using the ECL technique