Anti-CD137 antibody [BBK-2]
| Name | Anti-CD137 antibody [BBK-2] |
|---|---|
| Supplier | Abcam |
| Catalog | ab3169 |
| Prices | $384.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | BBK-2 |
| Applications | IHC-F FC ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Ectodomain of human 4-1BB recombinant protein |
| Description | Mouse Monoclonal |
| Gene | TNFRSF9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing MOLT4 cells stained with ab3169 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3169, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed MOLT4 cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/23277_CD137-Primary-antibodies-ab3169-2.jpg)
Overlay histogram showing MOLT4 cells stained with ab3169 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3169, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed MOLT4 cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
Distribution, function, and prognostic value of cytotoxic T lymphocytes in - Distribution, function, and prognostic value of cytotoxic T lymphocytes in
Laurent C, Muller S, Do C, Al-Saati T, Allart S, Larocca LM, Hohaus S, Duchez S, Quillet-Mary A, Laurent G, Brousset P, Valitutti S. Blood. 2011 Nov 17;118(20):5371-9.
CD137 is expressed in human atherosclerosis and promotes development of plaque - CD137 is expressed in human atherosclerosis and promotes development of plaque
Olofsson PS, Soderstrom LA, Wagsater D, Sheikine Y, Ocaya P, Lang F, Rabu C, Chen L, Rudling M, Aukrust P, Hedin U, Paulsson-Berne G, Sirsjo A, Hansson GK. Circulation. 2008 Mar 11;117(10):1292-301. doi:
4-1BB is expressed on CD45RAhiROhi transitional T cell in humans. - 4-1BB is expressed on CD45RAhiROhi transitional T cell in humans.
Garni-Wagner BA, Lee ZH, Kim YJ, Wilde C, Kang CY, Kwon BS. Cell Immunol. 1996 Apr 10;169(1):91-8.