All lanes : Anti-CD14 antibody (ab185538) at 1/1000 dilutionLane 1 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysateLane 2 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysateLane 3 : SW480 (Human colon adenocarcinoma cell line) cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-CD14 antibody (ab185538) at 1/200 dilutionLane 1 : SW480 (Human colon adenocarcinoma cell line) control lysateLane 2 : PNGase F treated SW480 (Human colon adenocarcinoma cell line) lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution