Anti-CD14 antibody [61D3]

• Supplier: Abcam
• Catalog: ab25625
• Prices: $379.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 61D3
• Applications: FC IHC-F IP B/N
• Species Reactivities: Human
• Antigen: The details of the immunogen for this antibody are not available
• Description: Mouse Monoclonal
• Gene: CD14
• Conjugate: Unconjugated

Product images

Human peripheral blood cells stained with ab25625 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab25625, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Effect of CD14 neutralisation by ab25625 on LPS-induced cytokine release from alveolar macrophages, ATII and TT1 cells. Cells were incubated for 1 hr with 10 µg/ml ab25625 prior to addition of 100 ng/ml LPS for 24 hrs. The corresponding IgG isotypes were used as negative controls.

Product References

Innate immune responses to bacterial ligands in the peripheral human lung--role - Innate immune responses to bacterial ligands in the peripheral human lung--role

Thorley AJ, Grandolfo D, Lim E, Goldstraw P, Young A, Tetley TD. PLoS One. 2011;6(7):e21827.

Bone marrow subsets differentiate into endothelial cells and pericytes - Bone marrow subsets differentiate into endothelial cells and pericytes

Reddy K, Zhou Z, Schadler K, Jia SF, Kleinerman ES. Mol Cancer Res. 2008 Jun;6(6):929-36.