Anti-CD151 antibody [11G5a]
| Name | Anti-CD151 antibody [11G5a] |
|---|---|
| Supplier | Abcam |
| Catalog | ab33315 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 11G5a |
| Applications | IHC-P IHC-F IP WB FC ICC/IF ICC/IF ELISA |
| Species Reactivities | Human |
| Antigen | The details of the immunogen for this antibody are not available |
| Description | Mouse Monoclonal |
| Gene | CD151 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab33315 staining human fibrosarcoma cells by ICC/IF. Cells were PFA fixed and blocked with 100% Image-iTTM signal enhancer for 30 minutes at 20°C. The primary antibody was used at 10µg/ml concentration and incubated with sample for 1 hour at 25°C. Alexa Fluor®555 conjugated donkey monoclonal to mouse (H+L) was used as the secondary antibody, diluted 1/500. See Abreview
See Abreview
ICC/IF image of ab33315 stained HT 1080 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33315, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96789, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Integrin-free tetraspanin CD151 can inhibit tumor cell motility upon clustering - Integrin-free tetraspanin CD151 can inhibit tumor cell motility upon clustering
Palmer TD, Martinez CH, Vasquez C, Hebron KE, Jones-Paris C, Arnold SA, Chan SM, Chalasani V, Gomez-Lemus JA, Williams AK, Chin JL, Giannico GA, Ketova T, Lewis JD, Zijlstra A. Cancer Res. 2014 Jan 1;74(1):173-87.
Proteomic analysis of microvesicles released by the human prostate cancer cell - Proteomic analysis of microvesicles released by the human prostate cancer cell
Sandvig K, Llorente A. Mol Cell Proteomics. 2012 Jul;11(7):M111.012914.
Comparative proteomic analysis of plasma membrane proteins between human - Comparative proteomic analysis of plasma membrane proteins between human
Zhang Z, Zhang L, Hua Y, Jia X, Li J, Hu S, Peng X, Yang P, Sun M, Ma F, Cai Z. BMC Cancer. 2010 May 14;10:206.
CD151, the first member of the tetraspanin (TM4) superfamily detected on - CD151, the first member of the tetraspanin (TM4) superfamily detected on
Karamatic Crew V, Burton N, Kagan A, Green CA, Levene C, Flinter F, Brady RL, Daniels G, Anstee DJ. Blood. 2004 Oct 15;104(8):2217-23. Epub 2004 Jul 20.