Flow cytometric analysis of Ramos cells (right histogram) labeling CD168 with ab170527 at 1/10 dilution, compared to a negative control cell (left histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of formalin fixed, paraffin embedded Human breast carcinoma tissue labeling CD168 with ab170527 at 1/50 dilution. Immunohistochemistry followed by peroxidase conjugation of the secondary antibody and DAB detection.
Anti-CD168 antibody - C-terminal (ab170527) at 1/100 dilution + Ramos cell line lysates at 35 µg