Anti-CD19 antibody
| Name | Anti-CD19 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab76591 |
| Prices | $359.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP WB ELISA |
| Species Reactivities | Human |
| Antigen | KLH conjugated synthetic peptide selected from the C terminal region of human CD19 |
| Description | Rabbit Polyclonal |
| Gene | CD19 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![CD19 was immunoprecipitated using 0.5mg Ramos whole cell extract, 5µg of Rabbit polyclonal to CD19 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Ramos whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab76591.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 61kDa; CD19](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/23683_ab76591-234719-IPVI004ab7659120m.jpg)
CD19 was immunoprecipitated using 0.5mg Ramos whole cell extract, 5µg of Rabbit polyclonal to CD19 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Ramos whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab76591.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 61kDa; CD19
Anti-CD19 antibody (ab76591) at 1/60 dilution + Jurkat cell line lysates at 35 µg
All lanes : Anti-CD19 antibody (ab76591) at 1/50 dilutionLane 1 : Non transfected 293 cell lysates Lane 2 : CD19 transfected 293 cell lysates Lysates/proteins at 2 µg per lane.