Anti-CD3 epsilon antibody [EP449E]
| Name | Anti-CD3 epsilon antibody [EP449E] |
|---|---|
| Supplier | Abcam |
| Catalog | ab52959 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP449E |
| Applications | WB IHC-P IP FC |
| Species Reactivities | Human |
| Antigen | A synthetic peptide corresponding to residues in cytoplasmic domain of human CD3 epsilon |
| Description | Rabbit Monoclonal |
| Gene | CD3E |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Human peripheral blood lymphocytes stained with ab52959 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab52959, 1/1000 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blo
![Anti-CD3 epsilon antibody [EP449E] (ab52959) at 1/10000000 dilution + Jurkat cell lysate at 10 µgSecondarygoat anti-rabbit HRP at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/24304_ab52959wb.gif)
Anti-CD3 epsilon antibody [EP449E] (ab52959) at 1/10000000 dilution + Jurkat cell lysate at 10 µgSecondarygoat anti-rabbit HRP at 1/2000 dilution
ab52959 at 1/250-1/500 dilution staining CD3 epsilon in human spleen by Immunohistochemistry, Paraffin embedded tissue.ab52959 at 1/250-1/500 dilution staining CD3 epsilon in human spleen by Immunohistochemistry, Paraffin embedded tissue.
ab52959 showing positive staining in Normal tonsil tissue.
ab52959 showing positive staining in Normal spleen tissue.
ab52959 showing negative staining in Normal brain tissue.
ab52959 showing negative staining in Normal kidney tissue.
ab52959 showing negative staining in Skeletal muscle tissue.
Product References
In situ characterization of intrahepatic non-parenchymal cells in PSC reveals - In situ characterization of intrahepatic non-parenchymal cells in PSC reveals
Berglin L, Bergquist A, Johansson H, Glaumann H, Jorns C, Lunemann S, Wedemeyer H, Ellis EC, Bjorkstrom NK. PLoS One. 2014 Aug 20;9(8):e105375.
Strong expression of TGF-beta in human host tissues around subcutaneous - Strong expression of TGF-beta in human host tissues around subcutaneous
Brattig NW, Racz P, Hoerauf A, Buttner DW. Parasitol Res. 2011 Jun;108(6):1347-54.