Anti-CD3 epsilon antibody [mAbcam97707]

• Supplier: Abcam
• Catalog: ab97707
• Prices: $376.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: mAbcam97707
• Applications: WB IHC-P
• Species Reactivities: Human
• Antigen: Recombinant fragment derived from within residues 1 - 100 of Human CD3 epsilon
• Description: Mouse Monoclonal
• Gene: CD3E
• Conjugate: Unconjugated

Product images

All lanes : Anti-CD3 epsilon antibody [mAbcam97707] (ab97707) at 5 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 3 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

IHC image of ab97707 staining CD3 epsilon in human normal tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97707, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX