Immunofluorescent staining of Human cell line A-431 shows positivity in nuclei. Recommended concentration of ab121435 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121435 at a 1/250 dilution staining CD300C in paraffin-embedded Human spleen tissue by Immunohistochemistry.
All lanes : Anti-CD300C antibody (ab121435) at 1/250 dilutionLane 1 : Vector only transfected HEK293T lysateLane 2 : CD300C transfected HEK293T over-expression lysate (Co-expressed with a C-terminal myc-DDK tag ~3.1 kDa) developed using the ECL technique