All lanes : Anti-CD300C antibody [EPR16470] (ab195811) at 1/1000 dilutionLane 1 : Mouse heart lysateLane 2 : Mouse kidney lysateLane 3 : Mouse spleen lysateLane 4 : Rat heart lysateLane 5 : Rat spleen lysateLane 6 : RAW 264.7 lysateLane 7 : PC12 lysateLane 8 : NIH 3T3 lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
All lanes : Anti-CD300C antibody [EPR16470] (ab195811) at 1/1000 dilutionLane 1 : Human fetal spleen lysateLane 2 : Human fetal brain lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Anti-CD300C antibody [EPR16470] (ab195811) at 1/1000 dilution + THP1 cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution