All lanes : Anti-CD32 antibody [9C6] (ab124408) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CD32 Lysates/proteins at 5 µg per lane.
Immunoprecipitation of CD32. Lane 1: Negative control, no IgG. Lane2: 2µg of ab124408 and 20µl (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed with lysate and incubated overnight. For each experiment, 500µl of DDK tagged CD32 overexpression lysates (at 1:5 dilution with HEK293T lysate) was used.
ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human breast tissue by Immunohistochemistry.
ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human colon tissue by Immunohistochemistry.
ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human lung tissue by Immunohistochemistry.
ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human ovary tissue by Immunohistochemistry.
ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human thyroid tissue by Immunohistochemistry.
ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human endometrium tissue by Immunohistochemistry.
ab124408, at 1/50 dilution, staining CD32 in paraffin-embedded Human prostate tissue by Immunohistochemistry.
ab124408, at 1/100 dilution, staining CD32 in COS7 cells transiently transfected with pCMV6-ENTRY CD32 by Immunofluorescence.