Anti-CD32 antibody [AT10]
| Name | Anti-CD32 antibody [AT10] |
|---|---|
| Supplier | Abcam |
| Catalog | ab41899 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | AT10 |
| Applications | B/N FC IHC-F IP B/N |
| Species Reactivities | Human, Dog |
| Antigen | K562 cell line |
| Description | Mouse Monoclonal |
| Gene | FCGR2A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing K562 cells stained with ab41899 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41899, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed K562 cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/24496_CD32-Primary-antibodies-ab41899-2.jpg)
Overlay histogram showing K562 cells stained with ab41899 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41899, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed K562 cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
IgG4 subclass antibodies impair antitumor immunity in melanoma. - IgG4 subclass antibodies impair antitumor immunity in melanoma.
Karagiannis P, Gilbert AE, Josephs DH, Ali N, Dodev T, Saul L, Correa I, Roberts L, Beddowes E, Koers A, Hobbs C, Ferreira S, Geh JL, Healy C, Harries M, Acland KM, Blower PJ, Mitchell T, Fear DJ, Spicer JF, Lacy KE, Nestle FO, Karagiannis SN. J Clin Invest. 2013 Apr;123(4):1457-74.
The response of human macrophages to beta-glucans depends on the inflammatory - The response of human macrophages to beta-glucans depends on the inflammatory
Municio C, Alvarez Y, Montero O, Hugo E, Rodriguez M, Domingo E, Alonso S, Fernandez N, Crespo MS. PLoS One. 2013 Apr 24;8(4):e62016.
Enhancing production and cytotoxic activity of polymeric soluble FasL-based - Enhancing production and cytotoxic activity of polymeric soluble FasL-based
Morello A, Daburon S, Castroviejo M, Moreau JF, Dechanet-Merville J, Taupin JL. PLoS One. 2013 Aug 26;8(8):e73375.
A panel of IgG1 b12 variants with selectively diminished or enhanced affinity for - A panel of IgG1 b12 variants with selectively diminished or enhanced affinity for
Moldt B, Schultz N, Dunlop DC, Alpert MD, Harvey JD, Evans DT, Poignard P, Hessell AJ, Burton DR. J Virol. 2011 Oct;85(20):10572-81.
C-reactive protein-derived peptide 201-206 inhibits neutrophil adhesion to - C-reactive protein-derived peptide 201-206 inhibits neutrophil adhesion to
El Kebir D, Zhang Y, Potempa LA, Wu Y, Fournier A, Filep JG. J Leukoc Biol. 2011 Dec;90(6):1167-75.
Internalization and coreceptor expression are critical for TLR2-mediated - Internalization and coreceptor expression are critical for TLR2-mediated
Bunk S, Sigel S, Metzdorf D, Sharif O, Triantafilou K, Triantafilou M, Hartung T, Knapp S, von Aulock S. J Immunol. 2010 Sep 15;185(6):3708-17.
Expression of adhesion and Fcgamma-receptors on canine blood eosinophils and - Expression of adhesion and Fcgamma-receptors on canine blood eosinophils and
Lilliehook I, Johannisson A, Hakansson L. Vet Immunol Immunopathol. 1998 Feb 27;61(2-4):181-93.
Expression of functional CD32 molecules on human NK cells is determined by an - Expression of functional CD32 molecules on human NK cells is determined by an
Metes D, Ernst LK, Chambers WH, Sulica A, Herberman RB, Morel PA. Blood. 1998 Apr 1;91(7):2369-80.
Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its - Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its
Greenman J, Tutt AL, George AJ, Pulford KA, Stevenson GT, Glennie MJ. Mol Immunol. 1991 Nov;28(11):1243-54.