Anti-CD32 antibody [AT10]
| Name | Anti-CD32 antibody [AT10] |
|---|---|
| Supplier | Abcam |
| Catalog | ab23336 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | AT10 |
| Applications | IP FC IHC-F |
| Species Reactivities | Human, Dog, Monkey |
| Antigen | Human K562 cell line |
| Description | Mouse Monoclonal |
| Gene | FCGR2A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing K562 cells stained with ab23336 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23336, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed K562 cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/24497_CD32-Primary-antibodies-ab23336-3.jpg)
Overlay histogram showing K562 cells stained with ab23336 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23336, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed K562 cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
Tonsil epithelial factors may influence oropharyngeal human immunodeficiency - Tonsil epithelial factors may influence oropharyngeal human immunodeficiency
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Cutting Edge: Antibody-mediated TLR7-dependent recognition of viral RNA. - Cutting Edge: Antibody-mediated TLR7-dependent recognition of viral RNA.
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Expression of adhesion and Fcgamma-receptors on canine blood eosinophils and - Expression of adhesion and Fcgamma-receptors on canine blood eosinophils and
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Expression of functional CD32 molecules on human NK cells is determined by an - Expression of functional CD32 molecules on human NK cells is determined by an
Metes D, Ernst LK, Chambers WH, Sulica A, Herberman RB, Morel PA. Blood. 1998 Apr 1;91(7):2369-80.
Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its - Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its
Greenman J, Tutt AL, George AJ, Pulford KA, Stevenson GT, Glennie MJ. Mol Immunol. 1991 Nov;28(11):1243-54.