Anti-CD3G antibody [EPR4517]

• Supplier: Abcam
• Catalog: ab134096
• Prices: $392.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR4517
• Applications: WB IP IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD3G (C terminal)
• Description: Rabbit Monoclonal
• Gene: CD3G
• Conjugate: Unconjugated

Product images

All lanes : Anti-CD3G antibody [EPR4517] (ab134096) at 1/50000 dilution (purified)Lane 1 : Jurkat cell lysateLane 2 : Human fetal thymus lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Anti-CD3G antibody [EPR4517] (ab134096) at 1/50000 dilution (purified) + Mouse thymus tissue lysate at 20 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-CD3G antibody [EPR4517] (ab134096) at 1/10000 dilution (unpurified)Lane 1 : Jurkat cell lysateLane 2 : Mouse thymus tissue lysateLysates/proteins at 10 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD3G with purified ab134096 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD3G with unpurified ab134096.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue labelling CD3G with unpurified ab134096.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon (lymphocytes) tissue labelling CD3G with unpurified ab134096.

Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human skeletal muscle tissue. Unpurified ab32362 shows negative staining.

Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human brain tissue. Unpurified ab32362 shows negative staining.

Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling CD3G with purified ab134096 at 1/1000. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.Control: primary antibody (1/1000) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

ab134096 (purified) at 1/40 immunoprecipitating CD3G in Jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

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