Anti-CD41 antibody [M148]
| Name | Anti-CD41 antibody [M148] |
|---|---|
| Supplier | Abcam |
| Catalog | ab11024 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | M148 |
| Applications | FC ICC/IF ICC/IF IP IHC-F |
| Species Reactivities | Human |
| Antigen | Tissue, cells or virus corresponding to Human CD41 |
| Description | Mouse Monoclonal |
| Gene | ITGA2B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC-Fr image of C41 staining on human blood film using ab11024 (1:1000). The sample was fixed using paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The slides were then blocked with 10% Donkey Serum for 1 hr at 24°C. ab11024 was diluted 1:100 using 0.1% TritonX with 0.1x PBS- 10% Donkeys and slides were incubated with the primary antibody for 4 hours at 24°C. The secondary antibody used was Donkey Polyclonal to rabbit IgG conjugated to Alexa Fuor 568 (1:1000)See Abreview
ICC/IF image of ab11024 staining of human platelet cells. The sections were incubated in 10% serum to block non-specific protein-protein interactions and in 0.2% triton X to permeabilise the cells. The sections were then incubated with ab11024 (1:400) for one hour at 20°C, followed by alexa 488 conjugated secondary antibody (green). Platelets were counterstained with rhodamine phalloidin to outline the cellular cytoskeleton (red).See Abreview
![Overlay histogram showing HL60 cells stained with ab11024 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11024, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed HL60 cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/24892_CD41Integrin-alpha-2b-Primary-antibodies-ab11024-27.jpg)
Overlay histogram showing HL60 cells stained with ab11024 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11024, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed HL60 cells used under the same conditions.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
Synovial membrane protein expression differs between juvenile idiopathic - Synovial membrane protein expression differs between juvenile idiopathic
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Proteomic characterization of human coronary thrombus in patients with ST-segment - Proteomic characterization of human coronary thrombus in patients with ST-segment
Alonso-Orgaz S, Moreno-Luna R, Lopez JA, Gil-Dones F, Padial LR, Moreu J, de la Cuesta F, Barderas MG. J Proteomics. 2014 Sep 23;109:368-81.
A monoclonal antibody binding to human medulloblastoma cells and to the platelet - A monoclonal antibody binding to human medulloblastoma cells and to the platelet
Jones D, Fritschy J, Garson J, Nokes TJ, Kemshead JT, Hardisty RM. Br J Haematol. 1984 Aug;57(4):621-31.