Anti-CD59 antibody [p282 (H19)]
| Name | Anti-CD59 antibody [p282 (H19)] |
|---|---|
| Supplier | Abcam |
| Catalog | ab79520 |
| Prices | $384.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | p282 (H19) |
| Applications | IHC-P FC WB |
| Species Reactivities | Human, Chimpanzee, Monkey, Monkey |
| Antigen | Full length protein corresponding to human CD59 |
| Description | Mouse Monoclonal |
| Gene | CD59 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab79520 at 10µg/ml staining CD59 by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded placenta sections).
![Overlay histogram showing Jurkat cells stained with ab79520 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79520, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/25366_CD59-Primary-antibodies-ab79520-2.jpg)
Overlay histogram showing Jurkat cells stained with ab79520 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79520, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Anti-CD59 antibody [p282 (H19)] (ab79520) at 1/2000 dilution + E. coli expressed mature human CD59 refolded at 0.5 µgSecondaryHRP-conjugated rabbit anti-mouse IgG polyclonal at 1/2000 dilutiondeveloped using the ECL techniquePerformed under non-reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/25367_ab79520-232150-ab79520wb.jpg)
Anti-CD59 antibody [p282 (H19)] (ab79520) at 1/2000 dilution + E. coli expressed mature human CD59 refolded at 0.5 µgSecondaryHRP-conjugated rabbit anti-mouse IgG polyclonal at 1/2000 dilutiondeveloped using the ECL techniquePerformed under non-reducing conditions.