Flow cytometry analysis of Human whole blood from buffy coat, staining CD69 with ab135933. Cells were stimulated with anti-CD3/CD28 overnight before isolation by a Ficoll density gradient. The sample was incubated with the primary antibody (1/100 in 1% BSA in PBS) for 1 hour at 4°C. Gating Strategy: Live lymphocytes See Abreview
Flow cytometry analysis of Cynomolgus monkey whole blood after red blod cell lysis, staining CD69 with ab135933. Cells were stimulated with PMA overnight and fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100 in 1% BSA in PBS) for 1 hour at 4°C. Gating Strategy: Live lymphocytesSee Abreview