Anti-CD74 antibody [LN2]
| Name | Anti-CD74 antibody [LN2] |
|---|---|
| Supplier | Abcam |
| Catalog | ab9514 |
| Prices | $390.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | LN2 |
| Applications | FC WB IHC-F IHC-P |
| Species Reactivities | Human |
| Antigen | SU-DHL-4 lymphoma cells |
| Description | Mouse Monoclonal |
| Gene | CD74 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab9514 staining human tonsil by IHC-P.
![Anti-CD74 antibody [LN2] (ab9514) at 5 µg/ml + Tonsil (Human) Whole Cell Lysate - adult normal tissue (ab29615) at 10 µgSecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/25677_CD74-Primary-antibodies-ab9514-2.jpg)
Anti-CD74 antibody [LN2] (ab9514) at 5 µg/ml + Tonsil (Human) Whole Cell Lysate - adult normal tissue (ab29615) at 10 µgSecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
ab9514 staining CD74 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with neutral buffered formalin and a heat mediated antigen retrieval step was performed using EDTA buffer pH 9.0. Samples were then blocked with 10% serum for 10 minutes at 20°C followed by incubation with the primary antibody at a 1/75 dilution for 30 minutes at 20°C. A HRP-conjugated rat anti-mouse/rabbit polyclonal was used undiluted as the secondary antibody.See Abreview
![Overlay histogram showing Raji cells stained with ab9514 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9514, 1/10 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/25679_CD74-Primary-antibodies-ab9514-6.jpg)
Overlay histogram showing Raji cells stained with ab9514 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9514, 1/10 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
Acquired resistance to anti-VEGF therapy in glioblastoma is associated with a - Acquired resistance to anti-VEGF therapy in glioblastoma is associated with a
Piao Y, Liang J, Holmes L, Henry V, Sulman E, de Groot JF. Clin Cancer Res. 2013 Aug 15;19(16):4392-403.
Macrophage migration inhibitory factor promotes tumor growth in the context of - Macrophage migration inhibitory factor promotes tumor growth in the context of
Arenberg D, Luckhardt TR, Carskadon S, Zhao L, Amin MA, Koch AE. Am J Respir Crit Care Med. 2010 Oct 15;182(8):1030-7. doi:
Expression of CD74, the receptor for macrophage migration inhibitory factor, in - Expression of CD74, the receptor for macrophage migration inhibitory factor, in
McClelland M, Zhao L, Carskadon S, Arenberg D. Am J Pathol. 2009 Feb;174(2):638-46.