Western blot detection of Cdc2 p34 phosphorylation using Saos-2 cells treated with hydroxyurea. Blots were probed with Cdc2 p34 (B-6): sc-8395 (A), p-Cdc2 p34 (Tyr 15)-R: sc-7989-R preincubated with cognate phosphorylated peptide (B) and p-Cdc2 p34 (Tyr 15)-R: sc-7989-R pre-incubated with identical unphosphorylated peptide (C).
p-Cdc2 p34 (Tyr 15): sc-7989. Western blot analysis of phosphorylated Cdc2 p34 expression in HeLa (A) and hydroxyurea-treated HeLa (B) whole cell lysates.
Western blot analysis of phosphorylated Cdc2 p34 expression in Saos-2 (A,C) and HeLa (B,D) whole cell lysates. Blots were probed with p-Cdc2 p34 (Tyr 15)-R: sc-7989-R (A,B) and p-Cdc2 p34 (Tyr 15)-R: sc-7989-R preincubated with its cognate phosphorylated peptide (C,D).
p-Cdc2 p34 (Tyr 15)-R: sc-7989-R. Western blot analysis of Cdc2 p34 phosphorylation in non-transfected 293: sc-110760 (A), human Cdc2 p34 transfected 293: sc-128282 (B) and HeLa (C) whole cell lysates.
p-Cdc2 p34 (Tyr 15): sc-7989. Western blot analysis of Cdk2 phosphorylation in non-transfected: sc-117752 (A) and mouse Cdk2 transfected: sc-119146 (B) 293T whole cell lysates.
p-Cdc2 p34 (Tyr 15): sc-7989. Western blot analysis of Cdc2 p34 phosphorylation in non-transfected 293: sc-110760 (A), human Cdc2 p34 transfected 293: sc-128282 (B) and HeLa (C) whole cell lysates.
p-Cdc2 p34 (Tyr 15)-R: sc-7989-R. Western blot analysis of Cdc2 p34 phosphorylation in non-transfected 293T: sc-117752 (A), mouse Cdc2 p34 transfected 293T: sc-119124 (B) and K-562 (C) whole cell lysates.
p-Cdc2 p34 (Tyr 15)-R: sc-7989-R. Western blot analysis of Cdc2 p34 phosphorylation in untreated (A) and lambda protein phosphatase (sc-200312A) treated (B) HeLa whole cell lysates.
p-Cdc2 p34 (Tyr 15)-R: sc-7989-R. Western blot analysis of Cdc2 p34 phosphorylation in HeLa whole cell lysate.
p-Cdc2 p34 (Tyr 15): sc-7989. Western blot analysis of Cdc2 p34 phosphorylation in non-transfected: sc-117752 (A) and human Cdc2 p34 transfected: sc-158363 (B) 293T whole cell lysates.
Western blot analysis of Cdc2 p34 phosphorylation in untreated (A,F), Hydroxyurea treated (B,G), nocodazole treated (C,H), Hydroxyurea and lambda protein phosphatase treated (D,I) and nocodazole and lambda protein phosphatase treated (E,J) HeLa whole cell lysates. Antibodies tested include p-Cdc2 p34 (Tyr 15)-R: sc-7989-R (A-E) and p-Cdc2 p34 (PSTAIRE): sc-53 (F-J).
p-Cdc2 p34 (Tyr 15)-R: sc-7989-R. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of subset of cells in seminiferous ducts.