Anti-CD82 antibody
| Name | Anti-CD82 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab66400 |
| Prices | $395.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human, Rat, Bovine |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human CD82 |
| Description | Rabbit Polyclonal |
| Gene | CD82 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-CD82 antibody (ab66400) at 1 µg/ml + Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ab66400 (1:80) staining CD82 in paraffin-embedded human tonsil tissue (left panel) using an automated system (Ventana Discovery). Right hand panel shows negative control (no primary antibody).Using this protocol there is strong membrane staining of follicular dendritic cells, and the FDC network forming the structure of germinal centres is clearly highlighted by this antibody. There is associated weak staining of smaller cells in the interfollicar areas which may be T cells.Sections were rehydrated and antigen retrieved in CC1 Cell Conditioning Buffer using Ventana Standard Retrieval programme. Slides were blocked in 3% H2O2 / 4 min / 37°C and incubated with ab66400 (1:80 dilution / 1 hour / 37°C). Sections then blocked (4mins / 37°C) and incubated with Dako swine anti-rabbit antibody (1:50, 28 min / 37°C). Staining was amplified and detected by incubation with Ventana Streptavidin ABC system (16 min / 37°C) and Ventana DAB map reagent (8 min / 37°C). Slides were counterstained with
ab66400 (1/50) staining CD82 in paraffin-embedded Mouse salivary gland tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. The secondary antibody was goat anti rabbit/mouse conjugated to HRP. For further experimental details please refer to abreview.See Abreview
Immunofluorescence analysis of HTR8/SVneo cell lines, staining CD82 (green) with ab66400. A FITC-conjugated anti-rabbit IgG (1/100) was used as the secondary antibody. Nuclei were detected by DAPI staining (blue).
Product References
The membrane scaffold CD82 regulates cell adhesion by altering alpha4 integrin - The membrane scaffold CD82 regulates cell adhesion by altering alpha4 integrin
Termini CM, Cotter ML, Marjon KD, Buranda T, Lidke KA, Gillette JM. Mol Biol Cell. 2014 May;25(10):1560-73.
Expression of CD82 in human trophoblast and its role in trophoblast invasion. - Expression of CD82 in human trophoblast and its role in trophoblast invasion.
Zhang Q, Tan D, Luo W, Lu J, Tan Y. PLoS One. 2012;7(6):e38487.