IHC image of ab58830 staining in Human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58830, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Lane 1 : Anti-CD82 antibody (ab58830) at 0.5 µg/mlLane 2 : Anti-CD82 antibody (ab58830) at 1 µg/mlLane 1 : A549 cell lysateLane 2 : A549 cell lysateLysates/proteins at 15 µg per lane.