Anti-CD82 antibody [TS82b]
| Name | Anti-CD82 antibody [TS82b] |
|---|---|
| Supplier | Abcam |
| Catalog | ab59509 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | TS82b |
| Applications | IHC-P WB IP FC |
| Species Reactivities | Rat, Human |
| Antigen | Jurkat and HEL cell lines |
| Description | Mouse Monoclonal |
| Gene | CD82 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab59509 staining CD82 in Rat placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilsed with 0.3% Triton X-100 and blocked with 3% serum for 60 minutes at 23°C; antigen retrieval was by heat mediation in 10mM citrate buffer pH6.0. Samples were incubated with primary antibody (1/200 in blocking buffer) for 12 hours at 4°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/100) was used as the secondary antibody.See Abreview
ab59509 staining CD82 (left) in Human peripheral blood neutrophils by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 1% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in PBS + 0.1% Triton-X 100 + 1% BSA) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Middle - LFA 1a, Right - merge.See Abreview
![Overlay histogram showing HeLa cells stained with ab59509 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59509, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/25891_CD82-Primary-antibodies-ab59509-7.jpg)
Overlay histogram showing HeLa cells stained with ab59509 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59509, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
![Overlay histogram showing HeLa cells stained with ab59509 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59509, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/25892_CD82-Primary-antibodies-ab59509-7.jpg)
Overlay histogram showing HeLa cells stained with ab59509 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59509, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
![Overlay histogram showing HeLa cells stained with ab59509 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59509, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/25893_CD82-Primary-antibodies-ab59509-7.jpg)
Overlay histogram showing HeLa cells stained with ab59509 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59509, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
Tetraspanins CD81 and CD82 facilitate alpha4beta1-mediated adhesion of human - Tetraspanins CD81 and CD82 facilitate alpha4beta1-mediated adhesion of human
Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, Chasis JA, Anstee DJ. PLoS One. 2013 May 21;8(5):e62654.
Identification of the tetraspanin CD82 as a new barrier to xenotransplantation. - Identification of the tetraspanin CD82 as a new barrier to xenotransplantation.
Saleh SM, Parhar RS, Al-Hejailan RS, Bakheet RH, Khaleel HS, Khalak HG, Halees AS, Zaidi MZ, Meyer BF, Yung GP, Seebach JD, Conca W, Khabar KS, Collison KS, Al-Mohanna FA. J Immunol. 2013 Sep 1;191(5):2796-805.
HIF-2 directly activates CD82 gene expression in endothelial cells. - HIF-2 directly activates CD82 gene expression in endothelial cells.
Nagao K, Oka K. Biochem Biophys Res Commun. 2011 Apr 1;407(1):260-5. doi: