Anti-CD83 antibody [1H4b]
| Name | Anti-CD83 antibody [1H4b] |
|---|---|
| Supplier | Abcam |
| Catalog | ab49324 |
| Prices | $379.00 |
| Sizes | 200 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 1H4b |
| Applications | IHC-P FC |
| Species Reactivities | Human |
| Antigen | Prokaryotic recombinant protein corresponding to 124 amino acids of the external N-terminus of the CD83 molecule |
| Description | Mouse Monoclonal |
| Gene | CD83 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab49324 staining CD83 in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in TRIS-EDTA buffer pH 9.0. Samples were incubated with primary antibody (1/50) for 30 minutes at 20°C. An undiluted HRP-conjugated Goat anti-mouse polyclonal was used as the secondary antibody.See Abreview
![Overlay histogram showing Jurkat cells stained with ab49324 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49324, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/25901_CD83-Primary-antibodies-ab49324-1.jpg)
Overlay histogram showing Jurkat cells stained with ab49324 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49324, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
Pro-inflammatory, Th1, Th2, Th17 cytokines and dendritic cells: a cross-sectional - Pro-inflammatory, Th1, Th2, Th17 cytokines and dendritic cells: a cross-sectional
Souto GR, Queiroz-Junior CM, de Abreu MH, Costa FO, Mesquita RA. PLoS One. 2014 Mar 26;9(3):e91636.
Distribution and density of CD1a+ and CD83+ dendritic cells in HPV-associated - Distribution and density of CD1a+ and CD83+ dendritic cells in HPV-associated
Kovalenko S, Lukashenko P, Romanovskaya A, Soldatski IL, Bakanov SI, Pfister H, Gerein V. Int J Pediatr Otorhinolaryngol. 2009 Feb;73(2):249-56. doi: